Ambrosetti is an Italian surname. Notable people with the surname include:
- Antonio Ambrosetti (1944–2020), Italian mathematician
- Bianca Ambrosetti (1914–1929), Italian gymnast
- Franco Ambrosetti, Swiss jazz musician
- Flavio Ambrosetti (1919–2012), Swiss jazz musician
- Gabriele Ambrosetti, Italian footballer and manager
- Juan Bautista Ambrosetti (1865–1917), Argentine archaeologist, ethnographer and naturalist
